Application Note: N/Protein Determination in Barley – Dumas and Kjeldahl method comparison

We have access to an application note focused on N/Protein determination in barley that compares both the Dumas and Kjeldahl methods using VELP Scientifica analysers available from a1-envirosciences GmbH.


Protein content of malting barley is important because it affects malting, brewing, and their end products. Kjeldahl determination is the commonly accepted method of protein estimation in barley, based on traditional wet chemistry.

An alternative to the classical Kjeldahl method is the Dumas combustion technique, innovative dry chemistry, easy to use and highly accurate.

Both the techniques are officially approved for the determination of the nitrogen and protein content in barley.

Kjeldahl Method

The modern Kjeldahl method consists in a procedure of catalytically supported mineralization of organic material in a boiling mixture of sulfuric acid and sulphate salt at digestion temperatures higher than 400 °C.

Dumas Method

The Dumas method starts with a combustion furnace (CF) to burn the sample, obtaining elemental compounds.


The obtained values fell within the expected protein range of the standard material certified by GAFTA, demonstrating the high performances of both type of VELP equipment, Kjeldahl system and Dumas analyser NDA 702.

Excellent repeatability is ensured with both techniques, as demonstrated by low RSD values.

To read more about N/Protein determination in barley and receive a copy of the full application note, email